Supplementary Figure 7: The effects of LIFR signaling on dormancy are mediated via STAT3 versus PI3K:mTOR or MAPK signaling.

(a) TCGA analysis of invasive breast carcinoma (Nature 2012 dataset) patient samples for mRNA down-regulation of LIFR, PIK3CA, and mTOR genes (n = 543 patients with no down-regulation, n = 32 patients with mRNA down-regulation). Logrank test. (b) TCGA analysis of invasive breast carcinoma (Nature 2012 dataset) patient samples for mRNA down-regulation of LIFR, MAPK3, and MAPK1 genes (n = 666 patients with no down-regulation, n = 52 patients with mRNA down-regulation). Logrank test. (c) Western blot for phospho-Akt, Akt, and loading control Actin protein levels in MCF7 cells treated with vehicle (water), 10 nM, 100 nM, or 1,000 nM BEZ235 (PI3K inhibitor) for 24 h. (d) mRNA levels for dormancy and quiescence-associated genes in MCF7 cells after 24 h treatment with BEZ235. Multiple t-tests with Holm-Sidak post-test. n = 3 biological replicates, each being an average from 3 independent experiments. (e) Western blot for phospho-Erk1/2, Erk1/2, and loading control Actin protein levels in MCF7 cells treated with vehicle (DMSO), 0.1 μM, 1 μM, or 10 μM AZD6244 (ERK1/2 inhibitor) for 24 h. (f) mRNA levels for dormancy and quiescence-associated genes in MCF7 cells after 24 h treatment with AZD6244. Multiple t-tests with Holm-Sidak post-test. n = 3 biological replicates, each being an average from 3 independent experiments. (g,h) mRNA levels for cancer stem cell-associated genes in MCF7 cells after 24 h treatment with (g) BEZ235 or (h) AZD6244. Multiple t-tests with Holm-Sidak post-test. n = 3 biological replicates, each being an average from 3 independent experiments. Unprocessed blots in Supplementary Fig. 9. Graphs represent the mean/group and error bars represent standard error of the mean (SEM). ^∗P < 0.05, ^∗∗P < 0.01, and ^∗∗∗P < 0.001.