Supplementary Figure 6: Hsp40 (DNAJA1), but not Hsp90, is involved in statin-induced mutp53 degradation, and reduced MVP by statins changes the binding partner of mutp53 from DNAJA1 to CHIP. | Nature Cell Biology

Supplementary Figure 6: Hsp40 (DNAJA1), but not Hsp90, is involved in statin-induced mutp53 degradation, and reduced MVP by statins changes the binding partner of mutp53 from DNAJA1 to CHIP.

From: DNAJA1 controls the fate of misfolded mutant p53 through the mevalonate pathway

Supplementary Figure 6: Hsp40 (DNAJA1), but not Hsp90, is involved in statin-induced mutp53 degradation, and reduced MVP by statins changes the binding partner of mutp53 from DNAJA1 to CHIP.The alternative text for this image may have been generated using AI.

(a) WB for Hsp90, Hsp90 client proteins (p53R175H, EGFR, Raf-1, and ErbB2), and β-actin or GAPDH using SK-Br-3 cells treated with DMSO (D) or lovastatin (4 μM). (b) Immunofluorescence for p53R175H using SK-Br-3 cells infected with lentiviral vectors encoding control or DNAJA1 shRNAs and treated with vehicle (ethanol) or leptomycin B (LMB, 50 nM). Scale bar, 50 μm.

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