Supplementary Figure 3: Active CDC-42 restricts accumulation of PKC-3 to PAR-3 clusters. | Nature Cell Biology

Supplementary Figure 3: Active CDC-42 restricts accumulation of PKC-3 to PAR-3 clusters.

From: Cortical forces and CDC-42 control clustering of PAR proteins for Caenorhabditis elegans embryonic polarization

Supplementary Figure 3: Active CDC-42 restricts accumulation of PKC-3 to PAR-3 clusters.

(a) The histograms show the distribution of intensity of PAR-3::GFP and PKC-3::GFP clusters during the establishment phases in zygotes expressing CDC-42Q61L, cdc-42(RNAi) zygotes, and cgef-1(RNAi) zygotes. Black and grey bars represent clusters with an R2 value from 2D Gaussian fitting over and below 0.6, respectively. Mean ± s.d. from n = 7 zygotes for wild type (EM phase) and cdc-42(RNAi), n = 6 for wild type (EE phase), and n = 5 each for other conditions. (b) Representative images of live zygotes expressing GFP::CDC-42, GFP::WSP-1CRIB, and GFP::PHPLC in wild-type, pkc-3(RNAi), nmy-2(ne3409), and cdc-42(RNAi) zygotes. All images are representative of 10 zygotes from 3 independent experiments. Scale bar, 5 μm. Cortical foci of GFP::CDC-42, GFP::WSP-1CRIB, and GFP::PHPLC were observed in pkc-3(RNAi) and nmy-2(ne3409) zygotes, where cortical contractility was low or inhibited, respectively. Cortical foci of GFP::PHPLC were observed in cdc-42(RNAi) zygotes.

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