Supplementary Figure 4: Cortical clustering is associated with efficient translocation of aPARs at the cortex. | Nature Cell Biology

Supplementary Figure 4: Cortical clustering is associated with efficient translocation of aPARs at the cortex.

From: Cortical forces and CDC-42 control clustering of PAR proteins for Caenorhabditis elegans embryonic polarization

Supplementary Figure 4: Cortical clustering is associated with efficient translocation of aPARs at the cortex.

(a) Fluorescence recovery after photo-bleaching (FRAP) analysis of PAR-3::GFP and PKC-3::GFP at the cortex during polarization. FRAP was performed on the anteromedial cortex of wild-type zygotes. Mean ± s.d. from at least 5 zygotes at each stage of polarization from 5 independent experiments. (b) Representative time-lapse images of live zygotes expressing NMY-2::Kate and PAR-3::GFP. Particle Image Velocimetry (PIV) analysis shows the orientation and magnitude of displacement of PAR-3::GFP and NMY-2::Kate in the PIV map (red arrows show NMY-2::Kate and blue arrows indicate PAR-3::GFP). The magnitude of PIV vectors is shown as a heat-map scale ranging from blue (lower value) to red (higher value). The cross-correlation of the orientation of PAR-3::GFP and NMY-2::Kate vectors depicts the magnitude of advective co-migratory behavior as a heat-map scale from blue (from 0 to −1) to red (+1). The times stated are with respect to the onset of symmetry breaking. Scale bar, 5 μm.

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