Supplementary Figure 5: Impact and regulation of the lncRNA on stem cell properties.
From: A regulated PNUTS mRNA to lncRNA splice switch mediates EMT and tumour progression
(a) (Top) Cell morphology of control and lncRNA overexpressing HMLE cells were assessed by phase-contrast microscopy and lncRNA-PNUTS overexpression was validated by RT–PCR. Scale bar, 50 μM. (Bottom) Histogram analysis related to FACS analysis presented in Fig. 6g. (b) Flow cytometry analysis of CD24 cell surface expression levels in MDA231-LM2 control (Scr-ShRNA) and shRNA-lncRNA-PNUTS. (c) Graphical abstract of the study. hnRNP E1 inhibits the alternative splicing of PNUTS by directly binding to the alternative splicing site. Loss of binding following hnRNP E1 knockdown, phosphorylation or its translocation to the cytoplasm allows for alternative splicing of PNUTS and thus generates lncRNA-PNUTS. This lncRNA competes for miR-205 and thus contributes to the EMT by allowing expression of mesenchymal markers such as Zeb1, normally targeted by this miRNA.
