Supplementary Figure 6: Primers and probes design.
From: A regulated PNUTS mRNA to lncRNA splice switch mediates EMT and tumour progression
(a) Design of the splice-specific primers used either in end-point RT–PCR or quantitative RT–PCR. Primers were designed to overcome primer competition in order to analyze the specific expression of either mRNA-PNUTS or lncRNA-PNUTS isoforms. (b) Design of the primers used in competitive end-point RT–PCR to analyze the relative expression between mRNA-PNUTS, lncRNA-PNUTS and preRNA-PNUTS on the same PCR reaction. (c) Validation of the reliability of the primer sets presented in a and b by end-point RT–PCR (d) Design of the probes used for Northern-blot experiments to discriminate mRNA-PNUTS from lncRNA-PNUTS. (e) Design of siRNA or shRNA used to selectively target the lncRNA isoform of PNUTS. Probes were designed to target the new exon11/exon12 splice junction specific to the lncRNA-PNUTS.
