Supplementary Figure 7: Methylated PKM2 restrains mitochondrial addiction to Ca2+ through IP3Rs.
From: PKM2 methylation by CARM1 activates aerobic glycolysis to promote tumorigenesis
(a) Western blot analysis of relative IP3R3 in MCF7 and MDA-MB-231 cells. (b) Western blot analysis of IP3R3 knockdown efficiency in MCF7 PKM2 KO and MDA-MB-231 cells. (c) The gating strategy of flow cytometry. (d) Representative images of parental MDA-MB-231, PKM2 KO, PKM2wt/shPKM1 and PKM2mut/shPKM1 cells after treatment with 5 μM XeB for 24 hours. Scale bars, 50 μm. In a,b, data represent one of the three independent experiments with similar results. Unprocessed original scans of blots are shown in Supplementary Figure 9.
