Figure 6: Haematopoietic ESL-1 preserves the CXCL12-producing bone marrow stroma.

(a) Whole-mount staining of sternal BM of Cxcl12^GFP mice transplanted with WT or Glg1^−/− bone marrow cells; n=4. Scale bar, 50 μm. (b) CXCL12 protein levels in the BM mice transplanted with WT or Glg1^−/− bone marrow cells; n=5. (c) Representative density plots showing osteoblasts (OB), CAR and endothelial cells (ECs) in the bone marrow of the BM of Cxcl12^GFP mice reconstituted with WT or Glg1^−/− BM, and treated or not with an anti-TGFβ antibody for 2 weeks, and quantification of their relative numbers (d); n=5 mice per group. Data are shown as mean±s.e.m.; *P<0.05; **P<0.01; ***P<0.001 as determined by Student’s t-test (b), or one-way analysis of variance with Turkey’s test (d).