Research articles

Here, structural analysis by cryoFIB-SEM and cryoET of virus-induced cell remodeling shows how phage SPP1 takes over the bacterial cytoplasmic space to confine stepwise assembly of virions starting from procapsid formation at the cellular membrane.

This study quantifies the emission impacts of operational efficiency improvements and constraints in China’s fast-evolving road freight sector, finding logistics enhancement a viable strategy for the sector’s decarbonization.

ADP-ribosylation regulates DNA repair, but its distinct forms have been difficult to distinguish. Here, the authors develop sensitive, modular antibodies to precisely detect mono- and poly-ADP-ribosylation and show that XRCC1 loss amplifies PARP1-dependent mono-ADP-ribosylation during DNA damage.

This study demonstrates that pristine graphene is intrinsically hydrophobic, regardless of the underlying substrate. The apparent hydrophilicity observed experimentally is shown to arise from signal cancellation by intercalated water molecules.

Here authors generate a multi-omics atlas of the perinatal Bama miniature pig brain, revealing a conserved developmental inflection point marked by large-scale transcriptomic and proteomic shifts associated with synaptogenesis and gliogenesis.

The study introduces manifold topological deep learning (MTDL) that extends topological deep learning to data on differentiable manifolds through de Rham-Hodge theory. MTDL outperforms competing methods on various biomedical imaging benchmarks.

Mutations in the follicle-stimulating hormone receptor (FSHR) cause spontaneous ovarian hyperstimulation syndrome (OHSS). Here, the authors report that estrildid finches naturally carry an analogous pathogenic mutation found in patients but avoid the syndrome by lowering a related receptor’s activity. Blocking this receptor successfully treats OHSS in mice.

Pooled single-cell perturbation screens can dissect genetic networks but face scalability issues in vivo. Here, the authors present MIC-Drop-seq, pairing high-throughput CRISPR in zebrafish with multiplexed scRNA-seq to enable scalable, whole-animal screening at single-cell resolution.

Metabolic reprogramming toward glycolysis is a hallmark of pathological cardiac hypertrophy, but the regulatory mechanisms remain unclear. Here the authors show that the adipokine, asprosin, drives hypertrophic metabolic remodeling by stabilizing PFKP and activating a YY1–asprosin–PFKP–PDK4–PDH axis.

Stabilizing 4d/5d metals as single atoms for catalysis is challenging due to their strong tendency to cluster. Here, the authors develop a hydrogen embrittlement-like assisted thermal activation strategy that prepares Ru-based single-atom catalysts, which achieve high performance in fuel cells.

Here the authors report that the π-basic cavity of pillar[n]arenes can effectively stabilize bromiranium intermediates generated during olefin halogenation, confining them in a controlled microenvironment. This strategy overrides the intrinsic Markovnikov preference, enabling highly selective anti-Markovnikov halogenation.

Pathological mechanisms underlying the onset and progression of PTSD (post-traumatic stress disorder) are not fully understood. Here authors show that stress-induced Sgk1 upregulation in basolateral amygdala neurons projecting to the hippocampus as a key mechanism underlying the delayed PTSD-like avoidance behavior in mice.

Systemic autoinflammatory disorders (SAID) represent a heterogeneous family of immune dysregulation disorders with overlapping clinical characteristics, making a precise and timely diagnosis challenging. Here, the authors collect samples from a group of patients diagnosed with autoinflammation of unknown origin, and by combining immunophenotyping, plasma proteomics, and machine learning, identify key immunological drivers of this condition with a signature that partially overlaps that of Still’s disease.

Bimerons are magnetic solitons that are topologically equivalent to skyrmions in in-plane magnetized systems. This study demonstrates the room-temperature creation of bimerons in Co₈Zn₈Mn₄ via femtosecond laser pulse excitation, revealing dynamic topological control and morphological transitions of these solitons.

How mutations shape signal processing in protein switches is not well defined. Here, the authors use GluePCA to profile thousands of PYL1 variants, uncovering modular control of sensitivity, basal activity and maximal response, novel inverter and band-stop mutations, and identifying stability‑driven mechanisms.

T cell immunoglobulin and mucin domain-containing protein 3 (TIM-3) is a protein that has been associated with regulatory processes in different immune cells. Here the authors demonstrate that TIM-3 is associated with regulation of type 2 innate lymphoid cells (ILC2) and use two different mouse allergy models to show how signalling through TIM-3 reduces allergic responses and inflammation.

The authors observe re-entrant superconductivity beyond a superconductor-to-insulator transition in a granular aluminum thin-film nanobridge, which they demonstrate functions as naturally occurring Josephson junction array. They argue that the re-entrant behavior is induced by the screening of electron charge, a many-body correlation effect.

CRISPR–Cas tools enable genome regulation but are often too large for efficient AAV delivery. Here, authors engineer a compact enhanced TnpB–ωRNA system (enTnpB) as a versatile genome regulation platform and develop a single-AAV regimen, ImmunAct, to activate endogenous cytokines and enhance cancer immunotherapy.

Habitat transitions can rewire microbial genomes. This study shows how a bacterial lineage crosses ecosystems either by acquiring genes or via hypermutability, where mutation bias—not selection—drives genome reduction and GC decline.

Authors measured eleven key antibiotic resistance genes (ARGs) in U.S. wastewater to gauge population‑level resistance. Results showed regional differences and links between higher ARG levels, social vulnerability indicators and international travel.