Figure 2: During dauer arrest, INS-35 is secreted into the intestinal lumen and is degraded.
From: Diapause is associated with a change in the polarity of secretion of insulin-like peptides
(a) Fluorescence images of INS-35::VENUS (left), ges-1p::mRFP (centre) and a merge (right) at the day 1 dauer stage. Arrows indicate the intestinal valve. Scale bar, 25 μm. (b) The top panels show images of INS-35::mRFP and AJM-1::GFP in the intestine at the day 1 dauer stage. Scale bar, 10 μm. The mean fluorescence intensity profile of AJM-1::GFP and INS-35::mRFP in the intestine is shown below. The dashed line indicates the cross section used to quantify fluorescence intensity indicated on the graph. (c) Western blot analysis using anti-GFP and anti-actin antibodies of extracts of ins-35p::ins-35::venus expressing worms: A, adult stage; D1, dauer stage at day 1; and D14, dauer stage at day 14. Images of the full blots reacted by anti-GFP or anti-actin antibodies are shown in Supplementary Fig. 6a,b.
