Figure 3: Photophysical properties of NIR-fluorophores.
From: Lysosome triggered near-infrared fluorescence imaging of cellular trafficking processes in real time
(a) Light absorption and emission spectra of compounds 2 and 5, and their photo-physical parameters. (b) Integrated off and on fluorescence states of 2 (5 × 10−6 M) in toluene, tetrahydrofuran (THF), dimethylformamide (DMF) and DMSO with TFA (red bars) and DBU (grey bars). (c) Plot of relative off and on integrated fluorescence versus solvent polarity values for toluene, THF, DMF and DMSO. (d) Comparative photobleaching of 1 × 10−7 M DMEM solutions of 2 (red line), lysotracker red (blue line) and pHrodo red (black line) with 150 W fibre optic delivered light 620(30) nm for 2 and 540(40) nm for lysotracker red and pH-rhodo red at 25 °C. (e) In vitro photobleaching of 2 (red), lysotracker red (blue line) and pHrodo red (black line) with maximum LED power using excitation filter 640(14) nm for 2 and excitation filter 563(9) nm for lysotracker red and pH-rhodo red.
