Figure 6: Identification of subcellular NIR-fluorescent on switch.
From: Lysosome triggered near-infrared fluorescence imaging of cellular trafficking processes in real time

CLSM fluorescent images showing lysosomal localization of the ‘on’ state of 2 in LAMP1-GFP-expressing HeLa cells (a) Cy5.5 channel; (b) GFP channel. (c) Three-dimensional image of overlaid Cy5.5 and GFP channels. (d) Zoom-in of the dashed box. Scale bars, 10 μm (a–c) and 2 μm (d).