Figure 7: Illustration of NIR-fluorescence response selectivity.
From: Lysosome triggered near-infrared fluorescence imaging of cellular trafficking processes in real time
(a) CLSM imaging of HeLa Kyoto cells following incubation with 2 (10 μM) for 2 h at 37 °C, DAPI nuclei staining and fixing. (b) The same set of cells imaged after buffer changed to pH 4.9, keeping the same laser power and PMT voltage. (c) The same set of cells after adjustment of microscope laser power and PMT voltage to obtain a non-saturated image. Red: 2; blue: DAPI stain. Scale bar, 10 μm.
