Figure 9: Z-axis projections of widefield 4D live-cell imaging of the uptake of 2 (10 μM) from HeLa Kyoto cells.
From: Lysosome triggered near-infrared fluorescence imaging of cellular trafficking processes in real time
Images were acquired in 25 focal planes every 1 min for 60 min. (a) Time lapse b/w images are shown for 15, 30, 40 and 60 min. (b) Red-coloured image at 60 min. (c) Fluorescence intensity quantification in two identical volumes around a selected cell (1) and in the extracellular environment (2). Scale bars, 10 μm.
