Figure 4: ET formation is ROS dependent.
From: Social amoebae trap and kill bacteria by casting DNA nets
(a) ROS signals (DHE staining, red) and S cells (AO staining, green) co-localize in AX4 slugs stimulated with K.p. See also Supplementary Movie 4 and Supplementary Fig. 5. (b) The ROS-sensitive OxyBurst Green (OBG)-coated beads were phagocytosed and oxidized by S cells, indicated by the fluorescence shift of ingested beads from red to yellow (arrowheads). The dashed lines in the lower gallery indicate the regions showed at higher magnification in the upper gallery. (c) Quantification of the signals for three phagocytosed and three non-phagocytosed beads over time. (d) Addition of catalase dose dependently decreased the levels of H2O2 (AUR assay), as well as extracellular DNA released (PI staining) from LPS-stimulated AX4 slug cells, n=3. Error bars: s.e.m. Scale bars, (a) 100 μm; (b) 5 μm.
