Table 1 X-ray crystallographic data collection and refinement statistics.

From: Sleeping Beauty transposase structure allows rational design of hyperactive variants for genetic engineering

Data collection

 Space group

I 21 21 21

 Cell dimensions

a, b, c (Å)

84.24, 113.98, 144.94

α, β, γ (°)

90, 90, 90

 Resolution (Å)

50–1.4 (1.50–1.40)*

 Rsym

0.081 (1.707)

 I/σI

17.9 (2.0)

 Completeness (%)

100.0 (100.0)

 Redundancy

11.2 (11.2)

 No. unique reflections

136,613 (25,318)

 CC(1/2)

1 (0.694)

Refinement

 Resolution (Å)

50–1.4

 No. reflections, all/free set

136,607/6,830

 Rwork/Rfree

0.171/0.195

 No. atoms

 

Protein

3,782

Ligand/ion

219

Water

721

 B-factors

25.40

Protein

22.40

Ligand/ion

39.60

Water

36.80

 R.m.s deviations

Bond lengths (Å)

0.020

Bond angles (°)

1.91

  1. *Highest resolution shell is shown in parenthesis.
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