Figure 1: Transcriptomic profiling of Fas2 expression. | Nature Communications

Figure 1: Transcriptomic profiling of Fas2 expression.

From: The cell adhesion molecule Fasciclin2 regulates brush border length and organization in Drosophila renal tubules

Figure 1

(a) Mean normalized Affymetrix signal±s.e.m. (N=4 tissue samples) showing the Fas2 spatial expression pattern across major tissues from both larval and adult Drosophila (flyatlas.org). (b) Overview of Drosophila anatomy with superimposed heat maps of the spatial expression pattern of Fas2. (c) Microarray and qPCR analyses of the dominant Fas2 isoforms across different tissues. The Affymetrix whole fly signal (dark grey bars) was set to an a.u. of 1, with head and tubule signals being expressed as a ratio to whole fly. The mean±s.d. qPCR expression ratios (light grey bars) largely reiterate the microarray data, albeit show much higher Fas2-RB transcript enrichment in MTs compared with the Affymetrix signal. *Significantly different (one-way ANOVA, P<0.05) compared with whole fly. (d) RNA-Seq data comparing Fas2 transcript levels across different tissues. The combined transcriptomic meta-analysis reveal that Fas2-RA, RB and RC are the main isoforms expressed in Drosophila, with isoform Fas2-RB being the dominant splice variant expressed in the MTs.

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