Figure 1: Dynamic changes in the number of KRT14^pos cells in the normal development and tissue repair upon injury.

(a) Immunofluorescence (IF) against KRT14 on bladders from E16.5, P5, Pw8 and Pw52 bladders. (b) Quantification of KRT14^pos cells at various developmental stages. Percentages are averages from three mice, and the total cells counted were 1,426, 1,669, 2,213 and 1,703 for E16.5, P5, Pw8 and Pw52, respectively. (c) IF against KRT14 and Ki67 on the bladder from an 8-week-old mouse before, and at 24 h after CPP injection. (d) Quantification of KRT14^pos cells at 6, 18, 24, 48, 120 and 240 h post CPP injection. (e) Quantification of urothelial cell proliferation at the above time points post CPP injection. (f) Quantification of proliferation of KRT14^pos and KRT14^neg urothelial cells during CPP-induced injury and repair. Numbers in b, d and e are expressed as mean of percentages over total urothelial cells±s.e.m. Numbers in f are mean of percentages over KRT14^pos or KRT14^neg cells±s.e.m. For b, d, e and f, two group comparison was performed using the Student’s t-test and the number of samples and P values can be found in the text, in Table 1 and in Supplementary Tables 1 and 2, respectively. For b, d and e, multiple comparison using Kruskal–Wallis test was also performed and P values were 0.0007, <0.0001 and 0.0001, respectively. Dash lines represent the basement membrane. Scale bars, 50 μm. N/T, not treated.