Figure 1: Activation of Nrf2 in HCC cells expressing phospho-mimetic p62.

(a) Enzymes involved in glucose and glutamine metabolism regulated by Nrf2. (b) Immunoblot analysis. FLAG-tagged p62 and its mutants were overproduced in the indicated HCC cells using an adenovirus vector. At 48 h after infection, cytosolic and nuclear fractions were prepared and subjected to immunoblot analysis with the specified antibodies. Data were obtained from three independent experiments.