Figure 1: Inability of a D2C to undergo RCA.
From: Programming a topologically constrained DNA nanostructure into a sensor
(a) Schematic illustration of a D2C made of CDNAi and rCDNAii, with a linking duplex of 24 bp. (b) Sequences of rD2C1, DP1 and DP2. Boxed nucleotides represent the 24-bp linking duplex. F: fluorescein-dT; R, adenosine ribonucleotide; Q, dabcyl-dT. (c) Synthesis of rD2C1 by circularizing linear DNAii over CDNAi as the template. Lane M, markers made of LDNAii, CDNAi and rCDNAii. Lane R: circularization mixture. (d) RCA reactions with gel-purified CDNAi, rCDNAii and rD2C1 using DP1 and DP2 as primers. Lane L, DNA ladders ranging from 1 to 10 kbp; RP, RCA product.
