Figure 2: Cleavage of an RNA-containing D2C by an RCD. | Nature Communications

Figure 2: Cleavage of an RNA-containing D2C by an RCD.

From: Programming a topologically constrained DNA nanostructure into a sensor

Figure 2: Cleavage of an RNA-containing D2C by an RCD.

(a) Restoration of RCA compatibility of an rD2C using an RCD. (b) Cleavage of rD2C1 by EC1, an E. coli-responsive DNAzyme. Concentration of E. coli: 105 cells ml−1. Reaction mixtures were analyzed by 10% denaturing PAGE. EC1M: a mutant EC1 that cannot be activated by E. coli. Both rCDNAii and CDNAi in rD2C1 were radioactively labelled with 32P to facilitate DNA visualization on the gel. Clv%: per cent cleavage.

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