Figure 3: 3′–5′ exonucleolytic activity of ϕ29DP on rCDNAii and rD2C1. | Nature Communications

Figure 3: 3′–5′ exonucleolytic activity of ϕ29DP on rCDNAii and rD2C1.

From: Programming a topologically constrained DNA nanostructure into a sensor

Figure 3: 3′–5′ exonucleolytic activity of ϕ29DP on rCDNAii and rD2C1.

Degradation of EC1-mediated cleavage product of rCDNAii (a,b) and rD2C1 (c) by ϕ29DP and PNK. Concentration of E. coli: 105 cells ml−1. Reaction mixtures were analyzed by 20% denaturing PAGE. LF, large DNA fragment; SF, small DNA fragment. M lanes contain various DNA markers as indicated. rCDNAii, both rCDNAii and CDNAi in rD2C1 were radioactively labelled with 32P to facilitate DNA visualization on the gel.

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