Figure 4: E. coli-dependent RCA reaction.
From: Programming a topologically constrained DNA nanostructure into a sensor
(a) RCA reactions of rD2C1 in the presence of E. coli (105 cells ml−1) analyzed using 0.6% agarose gel electrophoresis. Note every reaction also contained PNK and dNTPs. L, DNA ladders ranging from 1 to 10 kbp; RP, RCA product. (b) Determination of detection sensitivity through analysis of RP using 0.6% agarose gel electrophoresis. (c) Determination of detection sensitivity via the colourimetric assay enabled by PW17 peroxidase DNAzyme. (d) Analysis of assay specificity using the colourimetric assay. The gram-negative bacteria used were Serratia fonticola (SF), Achromobacter xylosoxidans (AX), Yersinia ruckeri (YR) and Hafnia alvei (HA). The gram-positive bacteria used were Leuconostoc mesenteroides (LM) and Pediococcus acidilactici (PA).
