Figure 1: Variability of endogenous protein abundance correlates with single-cell response to chemical inhibition.
From: Dichotomy of cellular inhibition by small-molecule inhibitors revealed by single-cell analysis
(a) IL-2 stimulation of the JAK–STAT pathway. (b) Single-cell pSTAT5 abundance in response to JAK inhibitor AZD1480. Inset, the coefficient of variation (CV) response to inhibition. (c) Single-cell contour plot of total STAT5 abundance and pSTAT5 in cells not treated with inhibitor, [IJAK]=0. Curve shows the resulting geometric mean of the pSTAT5 abundance conditioned on STAT5 abundance per cell. (d) Cell-to-Cell variability analysis reveals that the pSTAT5 response amplitude is correlated with STAT5 abundance. In addition, the sensitivity of cells to inhibition (IC50) exhibits a small negative correlation with STAT5 abundance (errorbars are standard deviation of experimental duplicates).
