Figure 4: Bach2 binds to the AP-1 motif containing regulatory regions of Th2 cytokine gene loci.
From: Bach2–Batf interactions control Th2-type immune response by regulating the IL-4 amplification loop
(a) Global patterns of Bach2 binding at the Th2 cytokine gene loci in the WT and Bach2-deficient naive cells cultured under neutral conditions for 5 days were determined using a ChIP-seq analysis. (b) The results of the ChIP assay with a quantitative PCR analysis of Bach2 binding in the WT naive CD4 T cells cultured under IL-2 conditions for 2 days; the results are presented relative to those of input DNA with the s.d. **P<0.01 (Student’s t-test; n=3). (c) Enriched Bach2-binding motifs in the naive CD4 T cells cultured under neutral conditions for 5 days. (d) The results of the pull-down assay of the binding of Bach2 to wild-type (WT) or mutant (Mut.) AP-1 consensus oligonucleotides in the effector CD4 T cell lysates. The data are representative of at least three-independent experiments with similar results. (e) The luciferase activity in 293 T cells transfected with an empty vector or Bach2, as well as a firefly luciferase reporter for AP-1, was unstimulated or stimulated with the phorbol ester 4-beta-phorbol 12-myristate acetate (PMA) (30 ng ml−1) for 16 h; the results are presented relative to the Renilla luciferase activity with the s.d. **P<0.01 (Student’s t-test; n=3).
