Figure 6: FZD6 mediates induction of the MES-associated phenotype and suppression of the Wnt signalling through TAK1. | Nature Communications

Figure 6: FZD6 mediates induction of the MES-associated phenotype and suppression of the Wnt signalling through TAK1.

From: A regulatory circuit of miR-125b/miR-20b and Wnt signalling controls glioblastoma phenotypes through FZD6-modulated pathways

Figure 6

(a) IB analysis on PN 23 and 17 spheres with or without FZD6 overexpression using indicated antibodies. (b) qRT–PCR analysis of PN-associated genes OLIG2 and SOX2, and MES-associated genes ALDH1A3 and CD44 in PN 23 spheres with or without FZD6 overexpression. (c) FZD6 expression promoted PN 23 spheres proliferation and self-renewal. (d) Ectopic expression of FZD6 promoted tumour growth of PN 23 spheres in GBM xenografts in the brain (n=5). Scale bar, 1.0 mm. (e) TOPFlash/FOPFlash assays on PN 23 with overexpressing FZD6 or control, with or without CaMKII inhibitor KN93 (5 μM), TAK1 inhibitor 5Z-7-oxo (5 μM), or a shRNA-NLK. (f,g) qRT–PCR analyses of the relative expression of miR-20b and miR-125b (f), and Wnt signalling target gene LEF1 (g) in PN 23 overexpressing FZD6, or a vector control, with or without CaMKII inhibitor KN93 (5 μM), TAK1 inhibitor 5Z-7-oxo (5 μM), or a shRNA-NLK. (h) sphere formation assays for PN 23 and 17 spheres with or without overexpression of FZD1, FZD3, and FZD5, respectively. (i) Summary of data shown in Supplementary Fig. 10. An overview of transmembrane receptors and Wnt ligands that affect FZD6 overexpression-induced phenotypes. Data were normalized to the control. *Relative inhibitory effects on indicated protein expression or cellular properties. Error bars (s.d.) represent the data of triplicate samples for each cell line or tumours from five individual mice in each group. *P<0.05, **P<0.01, paired two-way Student’s t-test. Data are representative from three independent experiments with similar results.

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