Table 1 Kinetic parameters obtained for the aminoacylation and ATP-PPi exchange reactions catalysed by LeuRSAt and AgnB2 at 28 °C at pH 7.4.

From: Structural characterization of antibiotic self-immunity tRNA synthetase in plant tumour biocontrol agent

Enzyme

Substrate

K m (μM)

kcat (s−1)

Aminoacylation

AgnB2

ATP

95.4±18.3

0.43±0.02

 

Leucine

28.7±7.3

0.35±0.03

 

tRNA

1.48±0.25

0.43±0.02

LeuRSAT*

ATP

396±83

0.33±0.02

 

Leucine

38±3.2

0.38±0.01

 

tRNA

0.94±0.19

0.36±0.02

ATP-PP i Exchange

AgnB2

ATP

1047±140

67±8.9

 

Leucine

12±2

73±3.6

LeuRSAT

ATP

357±22

87±1.5

 

Leucine

9.7±0.7

85±1.6

  1. All data in this table were average values obtained from three independent experiments with error±values representing s.d.
  2. *From reference Chopra, et al.23.
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