Figure 3: Probing electron transfer in vivo.
(a) Light-induced redox changes of cytochrome b in QiKO. Black, filled symbols, mildly reducing conditions; open symbols, after pre-illumination to get similar contents of pre-reduced and pre-oxidized haem b; red, strongly reducing conditions. Inset: reduction component on a smaller time scale. (b) Light-induced redox changes of cytochrome f in strongly reducing conditions. Black, WT; blue, QiKO, normalized on the photosystem I amount. Re-reduction of cytochrome f in QiKO is rate-limited by the re-oxidation of the bl haem. The reduction of cytochrome f in QiKO is biphasic, with the fast component being similar to the WT one (t1/2∼3 ms) (see inset), and the slow component being concomitant with the oxidation of bl (t1/2∼250 ms).
