Figure 1: Changes in expression levels of VCAM1 and α4 integrin.

(a) Tissue extracts were prepared from mouse spinal cords or whole brains on postnatal days 1–21 or in adulthood, subjected to SDS-polyacrylamide gel electrophoresis, transferred to a PVDF membrane and immunoblotted with an antibody against α4 integrin, VCAM1, CNPase, MBP or control actin. Data are representative of four experiments. (b) Rat oligodendrocytes and DRG neurons were co-cultured for 1–21 DIV, lysed and used for the respective immunoblottings. Data are representative of three experiments. (c) RT-PCR analysis was performed for α4 integrin, VCAM1 or control actin in differentiating oligodendrocytes (OLs) or neurons. Data are representative of two experiments. (d) Oligodendrocytes or neurons were lysed and used for immunoblottings. Data are representative of three experiments. (e) DIV2 co-cultures were co-stained with antibodies against VCAM1 (green) and NG2 (red; top row), VCAM1 (green), Tuj1 (red) and DAPI (middle row), or α4 integrin (green) and Tuj1 (red) (bottom row). PH, phase-contrast microscopy. Arrows indicate oligodendrocyte cell bodies. Data are representative of three experiments. Scale bar, 50 μm.