Figure 2: AQP1 reports gene expression over a large dynamic range.
From: Non-invasive imaging using reporter genes altering cellular water permeability
(a) Diffusion-weighted images (acquired at Δeff=398 ms, b=2,089 s mm−2) of CHO cells expressing AQP1 or GFP (control) and treated with varying doses of doxycycline to induce transgene expression. Scale bar, 3 mm. (b) Percent change in ADC of water in AQP1-expressing CHO cells (relative to control cells expressing GFP) as a function of doxycycline concentration, measured at different diffusion times. n=4 biological replicates. Error bars±s.e.m. (c) Levels of AQP1 expression on the membrane of CHO cells at different levels of doxycycline induction, estimated based on quantitative western blotting and relative expression of a co-transcribed GFP reporter, compared with values calculated from Monte Carlo simulations based on the ADC results in b. n≥3 biological replicates. Error bars±s.e.m.
