Figure 3: AQP1 expression is observable in mixed cell populations.
From: Non-invasive imaging using reporter genes altering cellular water permeability
(a) Illustration of the effect of an increasing fraction of AQP1-labelled cells in a tissue on the overall diffusivity of water. (b) Monte Carlo simulation predictions of change in ADC as a function of the fraction of cells expressing AQP1 in a mixed cellular lattice. (c) Top: diffusion-weighted MRI (acquired at Δeff=198 ms, b=2,334 s mm−2) of cells comprising AQP1-labelled cells mixed with GFP-labelled control cells in varying proportions. Bottom: image of mixed populations containing 0, 5 and 10% AQP1-expressing cells acquired using Δeff=398 ms, b=8,000 s mm−2, to maximize contrast for the low AQP1 fraction scenario, smoothed with a Gaussian filter (radius 1.5 pixels). Scale bars, 3 mm. (d) Percent change in ADC in mixed AQP1/GFP cell pellets as a function of the fraction of AQP1-expressing cells. N=4 biological replicates. Error bars±s.e.m.
