Figure 6: Reduced amounts of phosphorylated TYK2 and JAK2 in the patient’s fibroblasts.
From: Biallelic JAK1 mutations in immunodeficient patient with mycobacterial infection
(a,b) Primary fibroblasts from the patient or a healthy control were treated with the indicated concentrations of IFN-α (a) or IFN-γ (b) and protein extracts were subjected to immunoblotting. *shows non-specific protein species detected by the antibody. Arrowhead shows specific bands. Representative of three independent experiments (IFN-α 15’ and IFN-γ) or one experiment (IFN-α 5’). Fold change of band densitometry is indicated (numbers below bands and bar graphs in Supplementary Fig. 8).
