Figure 8: JAK1 pseudokinase domain is required for mediating IFN-γ signalling.
From: Biallelic JAK1 mutations in immunodeficient patient with mycobacterial infection
Stable clones of the JAK1-deficient Flp-In U4C fibrosarcoma cells, expressing JAK1WT, JAK1P733L/P832S, JAK1K908E, JAK1KinΔ, JAK1ΨKinΔ, JAK1ΨKinΔ/K908E or JAK1KinΔ/ΨKinΔ were exposed to 100 ng ml−1 IFN-α or 5 ng ml−1 of IFN-γ or medium (−) for 15 min. Cells expressing empty vector (e.v.) and parental, JAK1-competent, fibrosarcoma cells, 2fTGH, served as negative and positive controls, respectively. *Shows non-specific protein species detected by the antibody. Note that anti-phospho-JAK1 antibody cannot be used to analyse phosphorylation of JAK1KinΔ or JAK1KinΔ/ΨKinΔ, whereas anti-JAK1 antibody cannot detect JAK1ΨKinΔ, JAK1ΨKinΔ/K908E or JAK1KinΔ/ΨKinΔ, which lack relevant target epitopes. Representative of at least three independent experiments. Fold change of band densitometry is indicated (numbers below bands and bar graphs in Supplementary Fig. 10).
