Figure 5: YAP1–TEAD interaction is essential for WSS-induced motility.
From: Fluid shear stress activates YAP1 to promote cancer cell motility
(a) YAP1 protein expression in PC3 and LNCaP cells following transfection with a constitutively active form of YAP1 (S127A) or a TEAD-binding domain mutant (5SA/S94A). (b) Positivity of DsRed TEAD reporter in pEGFP-N1, YAP1 S127A and YAP1 5SA/S94A transfected PC3 cells indicates low to no activation by control and TEAD-binding mutant. (c) YAP1 S127A stimulated cell migration, whereas YAP1 5SA/S94A failed to increase movement relative to the pEGFP-N1 (Kruskal–Wallis one-way ANOVA, **P<0.001). (d) Quantification of motility with the YAP1–TEAD inhibitor verteporfin shows significant reduction in motility (n=3 independent experiments, Kruskal–Wallis one-way ANOVA, **P<0.001). (e) WSS-induced motility of PC3 cells was reduced with knockdown of individual TEADs (three independent experiments, all pairwise multiple comparison procedure by Dunn’s method, *P<0.05). (f) Knockdown of TEAD in combination shows reduction of motility to static levels with silencing of TEAD1-4 (three independent experiments, All pairwise multiple comparison procedure by Dunn’s method, *P<0.05). Error bars represent±s.e.m.
