Figure 1: Long-term glutaminolysis activation during amino acid restriction decreased cell viability. | Nature Communications

Figure 1: Long-term glutaminolysis activation during amino acid restriction decreased cell viability.

From: mTORC1 inhibition in cancer cells protects from glutaminolysis-mediated apoptosis during nutrient limitation

Figure 1

(a) U2OS (left panel) and HEK923 (right panel) cells were starved for all the amino acids (−AA) in the presence or absence of LQ or DMKG (2 mM) for 72 h (U2OS) or 144 h (HEK293). Representative microscopy images of the cells are shown for the indicated conditions. The scale bar represents 100 μm. (b) Proliferation curves for U2OS and HEK293 were determined upon −AA, in the presence or the absence of LQ and DMKG after 24–144 h. (c) Percentage of cell death was estimated using trypan blue exclusion assay upon LQ or DMKG treatment after 72 h for U2OS or 144 h for HEK293, as indicated. (d,e) A representative image of a clonogenic assay (left panel) and the quantification of the colonies formed in three independent experiments (right panel) are shown for U2OS (d) and HEK293 (e). (f,g) Percentage of cell death was estimated in cells depleted of GLS1 (siRNA GLS1) upon amino acids starvation either in the presence or the absence of LQ after 72 h for U2OS (f) and 144 h for HEK293 (g). (h,i) Percentage of cell death was estimated upon amino acids starvation either in the presence or the absence of LQ and BPTES (30 μM) after 72 h for U2OS and 144 h for HEK293 cells. Graphs show mean values±s.e.m. (n=3). *P<0.05 (Anova post hoc Bonferroni).

Back to article page