Table 1 SPR parameters of GST-Ufd2p and GST-Ufd2p NLM binding to K29-linked Ub2-GFP.

From: Ufd2p synthesizes branched ubiquitin chains to promote the degradation of substrates modified with atypical chains

 

Ka (1/Ms)

Kd (1/s)

KA (1/M)

KD (μM)

Ufd2p::Ub2-GFP

111

3.21e-5

3.46e6

0.29

Ufd2p NLM::Ub2-GFP

1.48e3

3.69e-3

4.01e5

2.49

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