Figure 1: TgNPT1 is a plasma membrane protein essential for parasite growth in DMEM but not in RPMI. | Nature Communications

Figure 1: TgNPT1 is a plasma membrane protein essential for parasite growth in DMEM but not in RPMI.

From: Cationic amino acid transporters play key roles in the survival and transmission of apicomplexan parasites

Figure 1: TgNPT1 is a plasma membrane protein essential for parasite growth in DMEM but not in RPMI.The alternative text for this image may have been generated using AI.

(a) Western blot analysis of TgNPT1-HA, probed with anti-HA antibodies. (b) Immunofluorescence assay of TgNPT1-HA (green) reveals partial colocalization with the plasma membrane marker SAG1 (red) (Pearson’s Correlation Coefficient mean±SD=0.81±0.04, n=6). The scale bar is 2?μm. (c) Western blot analysis demonstrating iTgNPT1-HA knockdown in the presence of ATc. Parasites were grown for 0, 3, 6, 12 and 24?h in the presence of ATc. GRA8 is a loading control. (d–f) Fluorescence growth assays for iTgNPT1 parasites (d,f) and iTgNPT1 parasites complemented with constitutively-expressed TgNPT1 (e); iTgNPT1/cTgNPT1), grown in DMEM (d,e) or RPMI (f), in the absence (black) or presence (red) of ATc. Growth is expressed as a percentage of that measured in parasites grown in the absence of ATc on the final day of the experiment. The data shown are averaged from three technical replicates (±s.d.), and are representative of those obtained in three biological replicates.

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