Figure 4: Impaired DNA replication can induce p21 expression. | Nature Communications

Figure 4: Impaired DNA replication can induce p21 expression.

From: DNA damage during S-phase mediates the proliferation-quiescence decision in the subsequent G1 via p21 expression

Figure 4

(a) Number of γH2AX foci per nucleus in cells treated with DMSO or aphidicolin for 24 h. DMSO, n=1527 cells; 0.3 μM Aph, n=775; 0.6 μM Aph, n=745. (b) Single-cell traces of p21-GFP expression after treatment of asynchronous cells with: DMSO, n=38; 0.3 μM aphidicolin, n=32; or 0.5 μM ATRi, n=40. All traces are aligned to S-phase exit. Black circles represent mitosis. Blue curves represent cells that enter S-phase, red curves represent cells that arrest in G1 and yellow curves represent cells that arrest in G2. (c) Number of γH2AX foci per nucleus in cells treated with DMSO or ATRi for 24 h. n=1527, DMSO; n=1420, 0.5 μM ATRi; n=1467, 1 μM ATRi. (d) CDK2 activity profiles in cells treated with 0.3 μM aphidicolin and: n=34, DMSO; n=32, p53; and n=42, p21 siRNA, aligned to S/G2. Black circles represent mitosis. Blue curves represent cells that enter S-phase, red curves represent cells that arrest in G1 and yellow curves represent cells that arrest in G2.

Back to article page