Figure 5: Skp2- and Cdt2-dependent pathways degrade p21 with different rates and timings. | Nature Communications

Figure 5: Skp2- and Cdt2-dependent pathways degrade p21 with different rates and timings.

From: DNA damage during S-phase mediates the proliferation-quiescence decision in the subsequent G1 via p21 expression

Figure 5

(a) Single-cell traces, coloured by p21-GFP intensity in G1, aligned to the G1/S transition. Four siRNA treatments are shown: n=65 cells, control; n=81, Skp2; n=37, Cdt2; n=25, Skp2 and Cdt2 co-depletion. Black circles represent mitosis. Note: only cells entering S-phase are shown. (b) Graph shows correlation between p21-GFP intensity and G1 length for each siRNA condition shown in (a): n=50 cells, control; n=54, Skp2; n=9, Cdt2; n=14, Skp2 and Cdt2 co-depletion. (c) Graph shows average p21-GFP nuclear level across FOV for four different siRNA treatments. Four FOVs are shown for control siRNA cells and two FOVs are shown for the other treatments, each curve is one FOV. Control siRNA is shown in blue, Skp2 siRNA is shown in green, Cdt2 siRNA is shown in yellow and double Skp2 and Cdt2 depletion is shown in red.

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