Figure 7: Arrest in response to serum starvation is not p21-dependent. | Nature Communications

Figure 7: Arrest in response to serum starvation is not p21-dependent.

From: DNA damage during S-phase mediates the proliferation-quiescence decision in the subsequent G1 via p21 expression

Figure 7

(a) Single-cell traces of p21-GFP levels aligned to mitotic exit (serum was withdrawn in the previous cycle); n=29 cells. Grey line marks 600 min time point used to define G1pm arrest. Red curves represent cells entering G1pm arrest, blue curves represent cells that enter S-phase. (b) Graph showing the percentage of cells arresting in G1 in three different mRuby-PCNA hTert-RPE1 cell lines (WT and two p21KO clones) after serum withdrawal for 24 h. Mean and s.d. of two independent FACS experiments are shown. (c) Model for how p21 propagates information on DNA damage in the mother cell to influence the proliferation-quiescence decision in daughter cells. At intermediate levels of p21 (purple curve) cells still cycle, albeit with a G1 delay that is p53-dependent but p21-independent.

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