Figure 3: Antibody-dependent enhancement of VP2-VLPs in primary B cells and in a monocytic cell line.
From: Extinct type of human parvovirus B19 persists in tonsillar B cells
The mean fluorescence intensity (MFI) of VP2-VLP-AF647 was measured by flow cytometry in tonsillar B cells from two seronegative individuals (a,b) as well as in U937 cells (c) after incubation with B19V-IgG+ (black solid line) or B19V-IgG- (black solid line, squares) total purified preparations. Error bars represent standard deviation (of four replicates). *Represents statistical significance (P<0.001) between positive and negative IgGs at a given concentration as calculated by Student’s t-test.
