Figure 4: Association of VP2-VLPs with EEA1-positive endosomes.
From: Extinct type of human parvovirus B19 persists in tonsillar B cells
Maximum intensity projections of serial confocal optical sections through Raji (a) and GM12878 cells (d) incubated overnight with VP2-VLP-AF647 (red) and treated with trypsin. White arrows represent enhanced cytoplasmic VLP localization in EEA1-positive endosomes (green). Differential interference contrast (DIC) merged with DAPI (blue) images are shown. Areas of localization (yellow) (b,e) and normalized fluorescent intensity profiles in zoomed areas (c,f) indicate VLP internalization. Scale bars, 2 μm.
