Figure 7: KDM3A/B directly erase H3K9 methylation and promote H3K4 methylation by MLL1.

(a) ChIP assays confirmed that the knockdown of KDM3A/B abolished KDM3A/B enrichments on the AXIN2 promoter in ALDH⁺HCP1 cells. (b) ChIP assays confirmed that the knockdown of KDM3A/B increased the levels of H3K9me2 on the AXIN2 promoter in ALDH⁺HCP1 cells. (c) ChIP assays showed that the knockdown of KDM3A/B did not affect β-catenin binding on the AXIN2 promoter in ALDH⁺HCP1 cells. (d) ChIP assays confirmed that the knockdown of KDM3A/B abolished KDM3A/B enrichments on the DKK1 promoter in ALDH⁺HCP1 cells. (e) ChIP assays confirmed that the knockdown of KDM3A/B increased the levels of H3K9me2 on the DKK1 promoter in ALDH⁺HCP1 cells. *P<0.05, **P<0.01, unpaired two-tailed Student’s t-test (n=3). (f) ChIP assays showed that the knockdown of KDM3A/B did not affect β-catenin binding on the DKK1 promoter in ALDH⁺HCP1 cells. *P<0.05, **P<0.01, unpaired 2-tailed Student’s t-test (n=3). (g) ChIP assays showed that the knockdown of KDM3A/B reduced the levels of H3K4me3 on the AXIN2 promoter in CSCs isolated HCP1 cells. (h) ChIP assays showed that the knockdown of KDM3A/B reduced the recruitment of MLL1 to the AXIN2 promoter in ALDH⁺HCP1 cells. (i) ChIP assays showed that the knockdown of KDM3A/B reduced the levels of H3K4me3 on the DKK1 promoter in ALDH⁺HCP1 cells. (j) ChIP assays showed that the knockdown of KDM3A/B reduced the recruitment of MLL1 to the DKK1 promoter in ALDH⁺HCP1 cells. (k) ChIP assays showed that KDM3A/B knockdown reduced BCL9/BCL9L occupancies on the AXIN2 promoter in ALDH⁺HCP1 cells. (l) ChIP assays showed that KDM3A/B knockdown reduced PYGO2 occupancies on the AXIN2 promoter in ALDH⁺HCP1 cells. (m) ChIP assays showed that KDM3A/B knockdown reduced BCL9/BCL9L occupancies on the DKK1 promoter in ALDH⁺HCP1 cells. (n) ChIP assays showed that KDM3A/B knockdown reduced PYGO2 occupancies on the DKK1 promoter in ALDH⁺HCP1 cells.