Figure 2: Targeting individual genomic sites with corresponding single sgRNAs.
From: CRISPR/Cas9 targeting events cause complex deletions and insertions at 17 sites in the mouse genome
Two genomic sites (B and C-1) were targeted independently with two individual sgRNAs (1 and 2). Four individual genomic sites (D-2, D-3, D-4(1) and F) were targeted with one sgRNA each. The deletion of two juxtaposed genomic sites (C-2/3) was generated in two steps. A single sgRNA-targeting site C-2 was injected into zygotes from mice carrying already a deletion in site C-3. The deletion of site C-3 was generated by TALEN19. B, Socs2; C and D, Wap; E, Csn1s1; F, Csn3. The red numbers refer to the sites being targeted in the respective experiments.
