Figure 2: h41 is responsible for RNase I-inhibition.
From: Specific inhibition of bacterial RNase T2 by helix 41 of 16S ribosomal RNA
(a) Secondary structure of E. coli 16S rRNAs. The secondary structure map was obtained from the Comparative RNA website and project (http://www.rna.ccbb.utexas.edu/). The 3′-major domain (U921-A1396, 476 bp) is encircled in pink. All RNA helices (h1–h45) are numbered and helices that were altered to Rpi sequences are circled with Rpi sequences. (b) Electrophoresis patterns of total RNA from KT103 cells harbouring 16S rRNA genes with various substituted 3′-major domains. (c) Narrowing down the inhibitory region within the 3′-major domain of Eco 16S rRNA by replacing RNA helices with the corresponding region of Rpi sequences. (d) Secondary structures of h41 of Eco, Sfi and Rpi. As compared with the Eco sequence, bases that are different in the Sfi and Rpi sequences are coloured purple and red, respectively. (b,c) A green dot in each lane represents the centre of the RNA mass (xcentre), which is used as the degradation index (see Methods). Locations of bands corresponding to 23S rRNA and 5S rRNA/tRNAs were defined as 100 and 0 (arbitrary units, a.u.), respectively, and are shown at the top of the gel.
