Figure 2: HBO1 is required for GG-NER but not TC-NER.
From: Phosphorylated HBO1 at UV irradiated sites is essential for nucleotide excision repair
(a) Depletion of HBO1 caused defects of unscheduled DNA synthesis (UDS) in HeLa cells. Cells were irradiated with 15 J m−2 ultraviolet and incubated for 3 h with EdU. Ultraviolet damage-dependent EdU incorporation of G1 cells was quantified and is shown as % of shCtl cells. Cells (n=1,500) from nine independent experiments; error bars indicate means±s.d. **P<0.01 (Student’s t-test). (b) Depletion of HBO1 did not affect recovery of RNA synthesis (RRS) after ultraviolet irradiation. HeLa shCtl or shHBO1 cells (left upper) and normal or UVsS-A fibroblasts (left bottom) were transfected with indicated siRNA. RRS assay was performed as described in Methods. Relative RNA synthesis is shown as % of mock irradiated. Depletion of CSB and HBO1 from HeLa and UVsS-A and HBO1 from fibroblasts was confirmed by western blotting (right). Cells (n=300) from three independent experiments; error bars indicate means±s.d.
