Figure 6: Anti-Ras DARPins inhibit intracellular Ras interactions with downstream effector molecules.
From: Structural and functional characterization of a DARPin which inhibits Ras nucleotide exchange
In an intracellular bioluminescent resonance energy transfer (BRET) assay, full-length K-Ras G12D-RLuc8 donor molecules were co-expressed with various Ras-binding or Ras-associated domains fused to GFP2 acceptors to trigger a BRET signal. (a) Diagrammatic format of donor, acceptor and competitor molecules. (b) Normalized BRET ratio (normalized to the negative control DARPin E3_5) generated in HEK293T cells transfected with plasmids expressing the indicated BRET pairs in the presence of different DARPins as competitors. Error bars represent the mean±s.d. (n=2).
