Figure 2: Gas chromatograms of acetylenic products of soldier beetle genes expressed in yeast. | Nature Communications

Figure 2: Gas chromatograms of acetylenic products of soldier beetle genes expressed in yeast.

From: The convergent evolution of defensive polyacetylenic fatty acid biosynthesis genes in soldier beetles

Figure 2

Fatty acid methyl esters were prepared from yeast expressing soldier beetle desaturases (a) CL10 and (b) empty vector pYES2 provided with linoleic acid. (c) CL4 and (d) empty vector provided with crepenynic acid. (e) CL4 and (f) empty vector provided with 9z12a14a-C18. Yeast was supplied exogenously with 0.2 mM fatty acids and analysis was conducted using method 1. Key to fatty acids: 9z12z-C16 palmitolinoleic; 9z12z-C18 linoleic; 9z12a-C18 crepenynic; 9z12a14z-C18 dehydrocrepenynic; 9z12a14a-C18 9Z-octadecen-12,14-diynoic; 9z12a14a16z/e-C18 9Z,16Z/E-octadecadien-12,14-diynoic acid. Underlined fatty acids are new products. Chromatograms a and b correspond to the ion current obtained by extraction of significant ions in the spectrum of methyl crepenynate—m/z 79, 93, 236. In a, the peak marked * eluting at 6.3 min was identified as 7Z,10Z-hexadecadienoic acid via analysis of the mass spectrum of the 4,4-dimethyloxazoline derivative and may arise from β-oxidation of linoleic acid (9z12z-C18) by yeast.

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