Figure 1: The R>L asymmetric distribution of Cerl2 mRNA is generated post-transcriptionally via its 3′-UTR.
From: Fluid flow and interlinked feedback loops establish left–right asymmetric decay of Cerl2 mRNA
(a) Whole-mount FISH analysis of Cerl2 mRNA with an exon probe in the node region of a mouse embryo at 2 pss. (b) Whole-mount FISH analysis as in (a) with a Cerl2 intron probe. (c) Quantitative FISH analysis of Cerl2 mRNA with an intron probe. Each data point represents the sum of the abundance of Cerl2 mRNA per embryo (a.u., arbitrary units) determined for crown cells on the left or right side of the node for an individual embryo at 1–3 pss. Dashed line indicates 5% fluctuated line from centre line (L=R). For each amount of Cerl2 RNA inside the nucleus, see also Supplementary Fig. S4. (d–g) Whole-mount in situ hybridization (WISH) analysis of Cerl2 mRNA with an intron probe (d,e) or an exon probe (f,g) in embryos cultured without (d,f) or with (e,g) 1 μg μl−1 actinomycin D (ACT) from 1 to 3 pss. (h) Whole-mount WISH analysis of lacZ mRNA in a Cerl2lacZ/lacZ embryo at 3 pss. (i) WISH analysis of lacZ mRNA in a Cerl2(lacZ) BAC transgenic embryo at 3 pss. (j–n) WISH analysis of Cerl2(sGFP) mRNA with an sGFP probe in embryos harbouring the indicated BAC transgenes at 3 pss. Scale bars: 50 μm. (o) Schematic representation of BAC transgenes and summary of the results obtained for the distribution of Cerl2(sGFP) BAC transcripts around the node.
