Table 1 E. coli strains and plasmids used in the 3-hydroxyacid pathway.

From: A platform pathway for production of 3-hydroxyacids provides a biosynthetic route to 3-hydroxy-γ-butyrolactone

Name

Relevant genotype

Reference

Strains

  

 DH10B

F− mcrA Δ(mrr-hsdRMS-mcrBC) ϕ80lacZΔM15 ΔlacX74 recA1 endA1 araD139Δ(ara, leu)7697 galU galK λ− rpsL nupG

Invitrogen

 ElectroTen-Blue

Δ(mcrA)183 Δ(mcrCB-hsdSMR-mrr)173 endA1 supE44 thi-1 recA1 gyrA96 relA1 lac Kanr [F′ proAB lacIqZΔM15 Tn10 (Tetr)]

Stratagene

 MG1655

F− λ− ilvG- rfb-50 rph-1

ATCC 700926

 MG0

MG1655 (DE3) ΔendA ΔrecA

30

 MG1

MG0 containing pET/bktB/hbd and pCDF/pct/tesB

This study

 MG2

MG0 containing pET/bktB/hbd and pCDF/prpE/tesB

This study

 MG3

MG0 containing pET/bktB/hbd and pCDF/ptb-buk/tesB

This study

 MG3B

MG0 containing pET/bktB/hbd and pCDF/(ptb-buk)B/tesB

This study

 MG4

MG0 containing pET/bktB/phaB and pCDF/pct/tesB

This study

 MG5

MG0 containing pET/bktB/phaB and pCDF/prpE/tesB

This study

 MG6

MG0 containing pET/bktB/phaB and pCDF/ptb-buk/tesB

This study

 MG6B

MG0 containing pET/bktB/phaB and pCDF/(ptb-buk)B/tesB

This study

 MG7

MG0 containing pETDuet-1 and pCDFDuet-1

This study

 MG0-GP

MG0 containing pHHD/ycdW-aceA-aceK

This study

 MG4-GDHP

MG4 containing pHHD/ycdW-aceA-aceK

This study

Plasmids

  

 pETDuet-1

ColE1(pBR322) ori, lacI, T7lac, AmpR

Novagen

 pCDFDuet-1

CloDF13 ori, lacI, T7lac, StrepR

Novagen

 pKVS45

p15 ori, tetR, Ptet, AmpR

56

 pHHD

Constructed by replacing the AmpR gene in pKVS45 with a KanR gene and eliminating the f1 origin of replication from the backbone

This study

 pET/bktB/hbd

pETDuet-1 harboring bktB from C. necator H16, and hbd from C. acetobutylicum ATCC 824

This study

 pET/bktB/phaB

pETDuet-1 harboring bktB and phaB from C. necator H16

This study

 pCDF/pct/tesB

pCDFDuet-1 harboring pct from M. elsdenii, and tesB from E. coli MG1655

This study

 pCDF/prpE/tesB

pCDFDuet-1 harboring prpE from S. typhimurium LT2, and tesB from E. coli MG1655

This study

 pCDF/ptb-buk/tesB

pCDFDuet-1 harboring a ptb-buk operon from C. acetobutylicum ATCC 824, and tesB from E. coli MG1655

This study

 pCDF/(ptb-buk)Bs/tesB

pCDFDuet-1 harboring a ptb-buk artificial operon from Bacillus subtilis, and tesB from E. coli MG1655

This study

 pHHD/ycdW-aceA-aceK

pHHD harboring the ycdW-aceA-aceK artificial operon

This study

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